We help CROs and biotech companies alike gain fresh insights on their drug candidates, enabling more efficient progress toward the clinic. The results below highlight some of the capabilities of 3D Bloom. To take a deeper dive, connect with our scientists by completing the short form at the link here.
CREATING 3D BLOOM MICROTISSUES IN 3 STEPS
(1) 3D Bloom is based on 2 biopolymers that are pipetted sequentially into U-bottom 96 well plates
(2) After mixing, the two 3D Bloom reagents crosslink in 30 minutes in a cell-friendly reaction to encapsulate cells in an ECM-like environment
(3) Media is added after 30 minutes and 3D culture begins
3D BLOOM VERSUS 3D SPHEROIDS
*Identical/parallel culture conditions for 21 days. Red is endogenous collagen (scale bars = 250 µm)
3D BLOOM VERSUS SCAFFOLDED 3D CULTURE PLATFORMS
(E.G. MATRIGEL OR COLLAGEN)
~100% Reproducibility Between 3D Bloom Batches
~50% Reproducibility Between Matrigel Batches
THE AUTOMATED 3D CULTURE PLATFORM
CellSpring has partnered with Tecan, the global leader in liquid handling robotics, to automate the 3D Bloom Platform.
To download the application note for creation of 3D Bloom Microtissues using the Tecan Freedom EVO, click here.
AUTOMATED PRODUCTION WITH THE TECAN FREEDOM EVO®
40k cells/well, Avg. 3DB diameter = 4.1mm, Avg. 3DB thickness = 70µm
3D Bloom lung cancer microtissues - one per well - produced uniformly by the Tecan Freedom EVO® in a 96 well plate.